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KMID : 0545120150250020174
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Journal of Microbiology and Biotechnology
2015 Volume.25 No. 2 p.174 ~ p.177
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α-Glucosidase Inhibitor Isolated from Coffee
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Kim Shin-Duk
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Abstract
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The potent α-glucosidase inhibitor (compound I) was isolated from coffee brews by the activity-based fractionation and identified as a β-carboline alkaloid norharman (9Hpyrido[ 3.4-b]indole) on the basis of mass spectroscopy and nuclear magnetic resonance spectra (1H NMR, 13C NMR, and COSY). The norharman showed a potent inhibition against α-glucosidase enzyme in a concentration dependent manner with an IC50 value of 0.27 mM for maltase and 0.41 mM for sucrase, respectively. A Lineweaver-Burk plot revealed that norharman inhibited α-glucosidase enzyme uncompetitively, with a Ki value of 0.13 mM.
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KEYWORD
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α-Glucosidase inhibitor, β-carboline, norharman, coffee, uncompetitive inhibitor
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